Part:BBa_K3670000:Design

XplA/B play a key role in the biodegradation of RDX.

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 936
  Illegal EcoRI site found at 1847
  Illegal EcoRI site found at 2288
  Illegal PstI site found at 1714
  Illegal PstI site found at 2775
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 936
  Illegal EcoRI site found at 1847
  Illegal EcoRI site found at 2288
  Illegal PstI site found at 1714
  Illegal PstI site found at 2775
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 936
  Illegal EcoRI site found at 1847
  Illegal EcoRI site found at 2288
  Illegal BglII site found at 1897
  Illegal BamHI site found at 2281
  Illegal BamHI site found at 2485
  Illegal XhoI site found at 2819
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 936
  Illegal EcoRI site found at 1847
  Illegal EcoRI site found at 2288
  Illegal PstI site found at 1714
  Illegal PstI site found at 2775
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 936
  Illegal EcoRI site found at 1847
  Illegal EcoRI site found at 2288
  Illegal PstI site found at 1714
  Illegal PstI site found at 2775
  Illegal NgoMIV site found at 148
  Illegal NgoMIV site found at 840
  Illegal NgoMIV site found at 1682
  Illegal NgoMIV site found at 1957
  Illegal NgoMIV site found at 2133
  Illegal NgoMIV site found at 2158
  Illegal NgoMIV site found at 2511
  Illegal AgeI site found at 942
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 751
  Illegal BsaI.rc site found at 1796
  

Design Notes

This sequence was obtained from GenBank and was designed by Seth-Smith, H.M.B. and Bruce, N.C.

Source

Rhodococcus rhodochrous strain 11Y

References

[1] S., & B. (2001). Rhodococcus rhodochrous strain 11Y adrenodoxin reductase-like (xplB) a - Nucleotide - NCBI. Retrieved October 25, 2020, from https://www.ncbi.nlm.nih.gov/nuccore/AF449421.1